[SLIM-Curve] SLIM Curve question

[Curtis Rueden]

Hi Steve,

I am CCing the slim-curve mailing list, which is the best place to ask
questions about SLIM Curve.

> While using the Fit Images function, histograms are generated for each
> parameter (A, x2, T, Z). The histograms are divided by quartiles which
> are indicated with dashed lines.
>
> My question is, is there a way to find or generate the values of where
> the quartiles are made in the histogram with SLIM Curve?

This quartiles are computed from the histogram like so:

https://github.com/slim-curve/slim-plugin/blob/master/src/main/java/loci/slim/histogram/HistogramDataNode.java#L165-L254

And then applied to a HistogramStatistics object:

https://github.com/slim-curve/slim-plugin/blob/5b12b4f64bd07318a47615b96215892336d706eb/src/main/java/loci/slim/histogram/HistogramDataGroup.java#L451-L460

You could override the quartile values using Java code, but then they
wouldn't really be "quartile" values anymore from a mathematical
perspective, since the actual distribution would not match what you define
as quartile boundaries.

Can you be more specific about what you are trying to achieve?

Regards,
Curtis

On Tue, Mar 17, 2015 at 8:27 PM, Steven Wu <scwu at umn.edu> wrote:

> Hello,
>
> I am using the SLIM Curve plugin in FIJI to process FLIM data files that
> were generated with SPCImage.
>
> While using the Fit Images function, histograms are generated for each
> parameter (A, x2, T, Z). The histograms are divided by quartiles which are
> indicated with dashed lines.
>
> My question is, is there a way to find or generate the values of where the
> quartiles are made in the histogram with SLIM Curve?
>
> Thanks for your help.
>
> Steve
>
> --
> Steven Wu, PhD
> University of Minnesota
> Department of Integrative Biology and Physiology
> Cancer and Cardiovascular Research Center
> 3-220 #27
> 2231 6th ST SE
> Minneapolis, MN 55455
> (612) 626-5585
>
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