[SLIM-Curve] parameter differences between FIJI an SPCImage5

[Paul Barber]

Dear Keliya,

Transient start should be near the rise of the transient.
Data start marks the data to be used for the fit and should be at the 
top of the transient or just after.
Transient end should be as large as possible, but exclude any strange 
data at the end to the time period.
The total time period should be at least 8 times the expected lifetime, 
if possible.
Threshold should be set to save time by not processing background pixels 
and its value will vary depending on your experiment. Set it at maybe 20 
photon counts.

You should fix these cursors and threshold for a given data set. Usually 
you are looking for a change in lifetime so the absolute lifetime value 
is not critical.

Hope that helps,
Paul.

On 27/10/2015 11:40, Keliya Bai wrote:
> Hello,
>
> The Photon Counting device I use, uses SPCImage5.0 to collect data (.sdt).
> I noticed that when opening files, FIJI Slim Curve usually sets the transient end time as 230 (display as indices), in comparison to SPCImage5.0 T2 value, usually sets to 240.
>
> I want to know what settings I should have, as well as what the Data Start value means.
>
> When I leave Transient End value and Data Start value alone, only changing Transient Start value, my lifetime value does not change.
> But when I do the other way round, the T(lifetime) value changes a lot.
> This makes me wonder what to set for these parameters (Transient Start, Data Start, Transient End, Threshold) for my Photon Counting documents.
>
> I'd be very much appreciated if you could help me with a better understanding of the logarithm behind the program, which I didn't get enough from the website, http://fiji.sc/SLIM_Curve.
>
> Thank you very much.
> Looking forward to your reply.
>
> Best wishes,
> Keliya Bai
>
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-- 
Dr Paul R Barber
CRUK/MRC Oxford Institute for Radiation Oncology