[SLIM-Curve] file formats

[KEVIN W ELICEIRI]

HI Ewan,

I would recommend for now not using SLIM-Curve and instead using TRI-2. We have lots of work on SLIM-Curve and its very incomplete in scripting and other areas. We don't currently have someone working on supporting this so for real data best to use TRI2.


best

k



Kevin W Eliceiri, PhD

PI and Director

Laboratory for Optical and Computational Instrumentation

University of Wisconsin at Madison

Investigator, Morgridge Institute for Research


________________________________
From: SLIM-Curve <slim-curve-bounces at imagej.net> on behalf of Ewan McGhee <e.mcghee at beatson.gla.ac.uk>
Sent: Thursday, March 8, 2018 11:01:37 AM
To: slim-curve at imagej.net
Subject: Re: [SLIM-Curve] file formats

Hi everyone,

Hopefully this mail list is still active and is just not very busy.

I came across the SLIM-Curve plugin for IMageJ/FII while looking up details of the Tri2 package as I have some FLIM data that I am having difficulty fitting.

When I saw that a macro-compatible FLIM fitting plugin as available I thought that this could be the best thing since sliced bread, but the only problem is that my data is in OME-TIFF format (each time bin is stored as an image in a z-stack) and it does not seem to be compatible with SLIM-Curve.

I was wondering if there is perhaps any chance that OME-TIFFs might be made a valid format? For example, the laser repetition frequency could be manually inputed and the  data order could be defined somehow.

I don’t know how easy this would be to implement but it would be a real saviour.

Cheers,

Ewan

—

Dr Ewan McGhee

Senior Scientific Officer
Beatson Advanced Imaging Resource (BAIR)
Beatson Institute for Cancer Research

e.mcghee at beatson.gla.ac.uk

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